DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.

DNA Microarray Analysis of the Central Carbohydrate Metabolism : 1 Glycolytic / Gluconeogenic Carbon Switch in the Hyperthermophilic Crenarchaeum 2 Thermoproteus tenax

Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data.

The hyperthermophilic, facultatively heterotrophic crenarchaeum Thermoproteus tenax was analyzed using a low-coverage shotgun-sequencing approach. A total of 1.81 Mbp (representing 98.5% of the total genome), with an average gap size of 100 bp and 5.3-fold coverage, are reported, giving insights into the genome of T. tenax. Genome analysis and biochemical studies enabled us to reconstruct its c...

Embden-Meyerhof-Parnas and Entner-Doudoroff pathways in Thermoproteus tenax: metabolic parallelism or specific adaptation?

Genome data as well as biochemical studies have indicated that--as a peculiarity within hyperthermophilic Archaea--Thermoproteus tenax uses three different pathways for glucose metabolism, a variant of the reversible EMP (Embden-Meyerhof-Parnas) pathway and two different modifications of the ED (Entner-Doudoroff) pathway, a non-phosphorylative and a semi-phosphorylative version. An overview of ...

The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.

Biochemical studies have suggested that, in hyperthermophilic archaea, the metabolic conversion of glucose via the ED (Entner-Doudoroff) pathway generally proceeds via a non-phosphorylative variant. A key enzyme of the non-phosphorylating ED pathway of Sulfolobus solfataricus, KDG (2-keto-3-deoxygluconate) aldolase, has been cloned and characterized previously. In the present study, a comparati...

Extensive Lysine Methylation in Hyperthermophilic Crenarchaea: Potential Implications for Protein Stability and Recombinant Enzymes

In eukarya and bacteria, lysine methylation is relatively rare and is catalysed by sequence-specific lysine methyltransferases that typically have only a single-protein target. Using RNA polymerase purified from the thermophilic crenarchaeum Sulfolobus solfataricus, we identified 21 methyllysines distributed across 9 subunits of the enzyme. The modified lysines were predominantly in alpha-helic...